原理介紹

無銅點(diǎn)擊化學(xué)反應(yīng)，即菌株促進(jìn)的疊氮炔環(huán)加成（SPAAC）是一種生物正交反應(yīng)，利用一對試劑，即環(huán)辛基和疊氮化合物，它們彼此有效地反應(yīng)，同時(shí)對羥基、氨基和硫醇等天然官能團(tuán)保持惰性。SPAAC能夠通過形成穩(wěn)定的三唑，在水和其他復(fù)雜的化學(xué)環(huán)境中標(biāo)記各種各樣的生物分子，而無需任何輔助試劑。在眾多已知的環(huán)辛基化合物中，二苯并環(huán)辛基化合物（DBCO）是一類具有相當(dāng)快的動(dòng)力學(xué)和良好的水緩沖穩(wěn)定性的試劑。在生理溫度和pH范圍內(nèi)，DBCO基團(tuán)不會(huì)與許多生物分子中天然存在的巰基、氨基或羥基發(fā)生反應(yīng)。DBCO-PEG4-琥珀酰亞胺酯是一種常用的通過氨基修飾將DBCO基團(tuán)引入生物分子的試劑。靶點(diǎn)科技分銷的ClickChemistryTools的DBCO-PEG4-NHS Ester，是高效率的DBCO 琥珀酰亞胺酯。

化學(xué)結(jié)構(gòu)

參考文獻(xiàn)

1. Wang X., et al. (2021). Equipping Natural Killer Cells with Cetuximab through Metabolic Glycoengineering and Bioorthogonal Reaction for Targeted Treatment of KRAS Mutant Colorectal Cancer. ACS Chem. Biol., 16 (4), 724-730.

2. Wiener, J., et al. (2020). Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics. Sci Rep., 10 (1), 1457.

3. Ahn, S.H., et al. (2020). Site-Specific 89Zr- and 111In-Radiolabeling and In Vivo Evaluation of Glycan-free Antibodies by Azide–Alkyne Cycloaddition with a Non-natural Amino Acid. Bioconjugate Chem., 31(4), 1177-87.

4. Pack, J., et al. (2020). Site-Specific Antibody Conjugation Strategy to Functionalize Virus-Based Nanoparticles. Bioconjugate Chem., 31(5), 1408-16.

5. Miller, A. L., et al. (2019). Rapid conjugation of nanoparticles, proteins and siRNAs to microbubbles by strain-promoted click chemistry for ultrasound imaging and drug delivery?. Polym. Chem., 10, 705-717.

6. Huynh, V., et al. (2018). Competitive Affinity Release for Long-Term Delivery of Antibodies from Hydrogels. Bioconjugate Chem., 57(13), 3406-10.

7. Nedrow-Byerst, J.R., et al. (2013). PSMA-targeted SPECT agents: mode of binding effect on in vitro performance. Prostate., 73(4), 355-62.