中文摘要：

肺微環(huán)境控制肺泡巨噬細(xì)胞（AMs）的穩(wěn)態(tài)和功能，而AMs是肺免疫的主要調(diào)節(jié)者，但其潛在機(jī)制，尤其是微生物組的作用，尚不清楚。在這里，我們使用Lyz2creEi24fl/fl小鼠，報(bào)告了巨噬細(xì)胞中EI24缺失會(huì)破壞AMs的穩(wěn)態(tài)，但通過(guò)代謝重編程增強(qiáng)其吞噬和炎癥反應(yīng)。因此，Lyz2creEi24fl/fl小鼠在肺部對(duì)病毒感染和腫瘤轉(zhuǎn)移表現(xiàn)出抵抗性。值得注意的是，腸道共生微生物通過(guò)TLR2/4信號(hào)通路上調(diào)AMs中的EI24表達(dá)。這些數(shù)據(jù)表明，微生物群通過(guò)上調(diào)AMs中的EI24以維持其穩(wěn)態(tài)，但會(huì)延緩其在肺中的免疫監(jiān)視功能。因此，我們的研究表明，刪除EI24可以增強(qiáng)基于巨噬細(xì)胞的免疫治療的抗病毒和抗腫瘤效果。

英文摘要：

Lung microenvironment controls the homeostasis and function of alveolar macrophages (AMs), the major regulators of lung immunity, but the underlying mechanisms, particularly the role of microbiota, remain unclear. Here, with Lyz2creEi24fl/fl mice, we report that EI24 deficiency in macrophages disrupts AMs homeostasis but enhances their phagocytosis and inflammatory responses via metabolic rewiring. Consequently, Lyz2creEi24fl/fl mice exhibit resistance to viral infection and tumor metastasis in lung. Notably, EI24 expression in AMs is upregulated by commensal microbiota through TLR2/4 signaling. These data demonstrate that microbiota upregulates EI24 in AMs to favor their homeostasis, but it retards their immune surveillance function in the lung. Our study thus indicates that deleting EI24 enhances anti-viral and anti-tumor effects of macrophage-based immunotherapy.

論文信息：

論文題目：Microbiota-induced EI24 improves homeostasis but impedes function of alveolar macrophages via metabolic regulation

期刊名稱：Nature Communications

時(shí)間期卷：17, Article number: 2227(2026)

在線時(shí)間：2026年1月31日

DOI： doi.org/10.1038/s41467-026-68466-5

產(chǎn)品信息：

貨號(hào)：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱：Clodronate Liposomes&Controlliposomes氯膦酸鹽脂質(zhì)體套裝

辦事處：靶點(diǎn)科技

Clodronate Liposomes氯膦酸鹽脂質(zhì)體在小鼠病毒感染和腫瘤轉(zhuǎn)移模型清除肺泡巨噬細(xì)胞和外周單核巨噬細(xì)胞。荷蘭Liposoma巨噬細(xì)胞清除劑ClodronateLiposomes見刊于Nature Communications：微生物群誘導(dǎo)的EI24通過(guò)代謝調(diào)控改善穩(wěn)態(tài)，但阻礙肺泡巨噬細(xì)胞功能。

Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體清除肺泡巨噬細(xì)胞和單核巨噬細(xì)胞的材料和方法：

In vivo cell depletion

Mice were first administered i.t. with 100?μL Clodronate or Control liposomes (Liposoma Technology) on day ?1 to deplete local AMs, and were subsequently injected i.v. with 100?μL Clodronate or Control liposomes at day 1 and with 5-day intervals to deplete potential monocyte-derived macrophages in lung69. To deplete T cells in vivo, mice were injected i.p. with 200?μg anti-CD4 (clone: GK1.5, Selleck) and 200?μg anti-CD8 (clone: 2.43, Selleck) at 7-day intervals.

材料和方法文獻(xiàn)截圖：